Techniques:
- Immunohistochemistry of FFPE tissue (classical IHC, OpalTM Multiplex, etc.)
- High quality RNA isolation from FFPE tissues & transcriptome analysis
- Isolation and biobanking of peripheral blood mononuclear cells (PBMCs)
- Isolation and biobanking of cerebrospinal fluid (CSF) cells [in preparation]
- Immunoprofiling via multi-color (> 14 parameters) spectral flow cytometry (Cytek® Aurora)
- Single-cell sequencing of PBMCs and CSF cells [in preparation]
- Spatial transcriptomics of FFPE tissues via the 10X Genomics Visium platform [in preparation]
- Digital immunoassays via Simoa® technology
- In-cell Western assays [in preparation]
- Classical cell culture including proliferation/viability assays, phagocytosis assay, oxidative stress assays, etc. [in preparation]
- Human microglia culture [in preparation]
- Ferrozine-based assay for iron quantitation of lysates
- DAB-enhanced Turnbull Blue staining for iron quantitation of FFPE tissue
- SYBR® Green qPCR, classical Western blotting